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KMID : 0368420070500020161
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Journal of Plant Biology
2007 Volume.50 No. 2 p.161 ~ p.166
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Dominance Relationship between Two Self-IncompatibleBrassica campestris Haplotypes in Response to CO2 Gas
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Hyun Ji-Young
Gothandam Kodiveri Muthukalianan
Baek Nam-Kwon
Wang Gongwei
Chung Yong-Yoon
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Abstract
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InBrassica, self-incompatibility (SI) can be overcome by CO2 application, an effective method for obtaining numerous inbred lines for F, commercial seed. We previously reported two different S-alleles ofBrassica campestris, S733 and S734, with extremely different degrees of susceptibility to this gas. In the current study, we raised a cross-population between those two genetic lines, and analyzed their reaction level of self-incompatibility to CO2 (RLSICO2). Here, all 40 of our progeny from the F1 cross-population were susceptible, maintaining high values of RLSICO2. This suggests that the susceptible line, S734, is dominant to the insusceptible line, S733. We also generated an F2 selfing-population of each crossed progeny, S733♀ S734♂ and S733♂ S734♀, to assess the RLSICO2 of each individual. PCR-RFLP analysis was performed to determine the S-genotype of the F2 population. The S734 allele segregated in a theoretical ratio of the dominant trait, and the RLSICO2 was consistent with the dominance relationship. Therefore, we have now demonstrated that high RLSICO2 in β.campestris is controlled by a dominant gene.
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KEYWORD
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dominant, PCR-RFLP, reaction level of self-incompatibility, Slocus receptor kinase
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